ABSTRACT
0.05).CONCLUSION: The PRF-mixed group showed more bone formation than the porcine cancellousbonegroup (TS-GBB), butthere was a no significant difference. The PRF may not lead to enhanced bone healing when grafted with the porcine cancellous bone.
Subject(s)
Animals , Rabbits , Bone Regeneration , Fibrin , Osteogenesis , Skull , Transplantation, Heterologous , TransplantsABSTRACT
A calcifying epithelial odontogenic tumor (CEOT) was first described as a separate entity in 1955 by Pindborg, and has since been referred to as Pindborg tumor. CEOT is characterized by the presence of squamous-cell proliferation, calcification and amyloid deposits, and accounts for only 1% of all odontogenic tumors. CEOT is a benign, though occasional locally invasive, slow-growing neoplasm. It is located either intraosseously or extraosseously, and is usually associated with an unerupted permanent tooth. A 24 year-old female visited our clinic, presenting with a palatal swelling and intra-oral ulcer. After an incisional biopsy, the lesion was confirmed to be odontogenic tumor. A tumor resection and reconstruction surgery with tongue flap were performed.
Subject(s)
Female , Humans , Biopsy , Jaw Neoplasms , Odontogenic Tumors , Palate , Plaque, Amyloid , Skin Neoplasms , Tongue , UlcerABSTRACT
Subject(s)
Animals , Humans , Mice , Abdominal Fat , Adipose Tissue , Adult Stem Cells , Alkaline Phosphatase , Anesthesia, Local , Biology , Bone and Bones , Bone Marrow , Bone Regeneration , Calcium , Durapatite , Enzyme-Linked Immunosorbent Assay , Mesenchymal Stem Cells , Mice, Nude , Microscopy, Fluorescence , Osteoblasts , Osteocalcin , Osteocytes , Osteogenesis , Osteopontin , Stem Cells , Stromal CellsABSTRACT
Subject(s)
Animals , Rats , Bone and Bones , Bone Marrow , Bone Regeneration , Hydrogels , Hydrogels , Mesenchymal Stem Cells , Osteoblasts , Osteogenesis , Polyesters , Polyethylene Glycols , TransplantsABSTRACT
Maxillary sinus lift and bone graft are used to reconstruct atrophic maxilla molar area for endosseous dental implants. Many different grafting materials and techniques can be used for maxillary sinus bone graft. Bio-Oss(R) has been proposed as bone substitute and successfully utilized as osteoconductive filler. Platelet rich plasma (PRP) is an autologous material with many growth factors, such as PDGF, TGF-beta, IGF, VEGF, facilitating bone healing process. And Platelet poor plasma (PPP) is the by-product in procedure of producing PRP. Six rabbits were used as experimental animal. Both maxillary sinus were grafted with Bio-Oss(R) and PRP, and Bio-Oss(R) and PPP. Rabbits were sacrificed at 4, 8 and 12 weeks. The grafting sites were evaluated by histomorphometric analysis. As a result, using PRP showed excellent bone formation in the early stage, but no further significant effect after that. In late stage, the ability of bone formation of using PRP was even worse than using PPP. The further studies need to be considered in this case.
Subject(s)
Animals , Rabbits , Blood Platelets , Bone Substitutes , Dental Implants , Intercellular Signaling Peptides and Proteins , Maxilla , Maxillary Sinus , Molar , Osteogenesis , Plasma , Platelet-Rich Plasma , Transforming Growth Factor beta , Transplants , Vascular Endothelial Growth Factor AABSTRACT
Subject(s)
Adult , Humans , Adipocytes , Adipose Tissue , Adult Stem Cells , Alkaline Phosphatase , Anesthesia, Local , Biology , Bone Marrow , Cell Count , Chondrocytes , Collagen Type I , Durapatite , Integrin-Binding Sialoprotein , Lipectomy , Mesenchymal Stem Cells , Microscopy, Fluorescence , Myoblasts , Osteoblasts , Osteocalcin , Osteocytes , Osteogenesis , Pluripotent Stem Cells , Stem Cells , Tissue EngineeringABSTRACT
Subject(s)
Blotting, Western , Fibroblasts , Heat-Shock Proteins , Heat-Shock Response , HSP27 Heat-Shock Proteins , Immunohistochemistry , Osseointegration , Osteoblasts , Primary Cell Culture , Proteins , Staphylococcal Protein AABSTRACT
PURPOSE: This study was done to know the usefulness of fractal analysis when evaluating the radiologic changes after decompression on jaw bone cystic lesions using fractal analysis. MATERIALS AND METHODS: 30cases of cystic lesions were followed up after decompression. Panoramic image was used to observe radiologic changes around the cystic lesion. The part of the panoramic image which showed radiologic change was defined as region of interest(ROI); The fractal dimension of the ROI was calculated using box-counting method. RESULTS: Using sign-rank test, there was a statistically significant difference in fractal dimensions after decompression therapy(P<;0.0001). The fractal dimensions statistically increased after decompression(the median of D:0.12). CONCLUSIONS: The ROI after decompression showed higher fractal dimensions which offer the objective proof of the bone healing around cystic lesions after decompression treatment.
Subject(s)
Bone Cysts , Decompression , Fractals , JawABSTRACT
Subject(s)
Animals , Humans , Mice , Rats , Bone Regeneration , Brain , Connective Tissue , Extracellular Matrix , Fibronectins , Mesenchymal Stem Cells , Mice, SCID , Mice, Transgenic , Models, Animal , Neurons , Osteoblasts , Osteogenesis , Publications , Stem Cells , Tissue DonorsABSTRACT
Subject(s)
Cadmium , Cell Line , Cell Line, Transformed , Cell Survival , Chromium , Heat-Shock Proteins , HSP27 Heat-Shock Proteins , Ions , Molecular Chaperones , Osteoblasts , Osteogenesis , TitaniumABSTRACT
Subject(s)
Animals , Humans , Rats , Alveolar Ridge Augmentation , Durapatite , Factor VIII , Fibrin Tissue Adhesive , Fibrin , Fibrinogen , Hemostasis , Polymers , Skin , Subdural Space , Surgery, Oral , Sutures , Thrombin , Tissue Adhesives , Tooth Extraction , Transplants , Wound HealingABSTRACT
Subject(s)
Adult , Humans , Anesthetics , Anesthetics, Local , Arterioles , Blood Glucose , Dental Clinics , Epinephrine , Glucose , Homeostasis , Lidocaine , Meals , Surgery, OralABSTRACT
Bone grafts are widely used in the reconstruction of osseous defects in the oral and maxillofacial region. Autogenous bone grafts are considered the gold standard in grafting of the oral and maxillofacial region, because of its osteoconductive and osteoinductive properties. Mandibular symphysis & ascending ramus bone graft have been used more frequently because of easy surgical access, reduced operative time, and following minimal morbidity. However, even though the frequent use of the anterior part of ascending ramus and the different regions of mandible, rare of the reports provide information about the quantity of bone available in this donor site. So this study was taken to evaluate & quantify the amount of bone graft material in the anterior ascending ramus regions. This study was made on 36 samples of CT image. In 3D volume image, imaginary osteotomy & segmentation were done and the dimensions and volume of the bone grafts were measured and evaluated. the average volume of the graft materials obtained from the ascending ramus was 3656.83+/-108.19mm3, and the average dimensions of graft materials were (33.68+/-0.48)x(34.92+/-0.51)x(15.96+/-0.27)x(9.05+/-0.27)mm.
Subject(s)
Humans , Autografts , Bone Transplantation , Mandible , Operative Time , Osteotomy , Tissue Donors , TransplantsABSTRACT
This study was to analyze the changes of levels of alkaline phosphatase before and after enucleation of jaw cysts combined with bone grafting, and to evaluate biochemically the effectiveness of the early detection of bone healing and infection as a prognostic marker. Eighteen patients (13 males, 5 females) with cystic lesions of the jaws were divided into two groups. The bone graft group underwent enucleation and bone graft. The control group underwent only enucleation. Both groups were measured levels of ALP before surgery, and plus-minus 4 weeks postoperatively. The more discriminating results were obtained in the bone graft group. The results were as follows : 1. Levels of ALP after enucleation of jaw cysts were decreased in all patients with and without bone graft. 2. The bone graft group showed more marked decrease in variation of levels of ALP than the control group.(p=0.008) This should be considered as a result of increased osteoblastic activity and new bone formation. 3. Such variation could be used as a prognostic marker for bone healing after cyst operation. In the cost/benefit ratio, measurement of ALP activity could be useful as a convenient procedure in routine clinical practice.
Subject(s)
Humans , Male , Alkaline Phosphatase , Bone Transplantation , Jaw Cysts , Jaw , Odontogenic Cysts , Osteoblasts , Osteogenesis , TransplantsABSTRACT
Subject(s)
Child , Humans , Decompression , Dentigerous Cyst , Depression , Jaw , Paresthesia , Tooth , TractionABSTRACT
Estrogen may promote osteoblast/osteocyte viability by limiting apoptotic cell death. We hypothesize that hsp27 is an estrogen- regulated protein that can promote osteoblast viability by increasing osteoblast resistance to apoptosis. The purpose of this study was to determine the effect of estrogen treatment and heat shock on TNF alpha- induced apoptosis in the MC3T3-E1 cell line. Cells were treated with 0 - 100 nM 17betaestradiol (or ICI 182780) for 0 - 24 hours before heat shock. After recovery, apoptosis was induced by treatment with 0 - 10 ng/ml TNF alpha. Hsp levels were evaluated by Northern and Western analysis using hsp27, hsp47, hsp70c and hsp70i - specific reagents. Apoptosis was revealed by in situ labeling with Terminal Deoxyribonucleotide Transferase (TUNEL). A 5 - fold increase in hsp27 protein and mRNA was noted after 5 hours of treatment with 10 - 20 nM 17beta estradiol prior to heat shock. Increased abundance of hsp47, hsp70c or hsp70i was not observed. TUNEL indicated that estrogen treatment also reduced (50%) MC3T3-E1 cell susceptibility to TNF alpha-induced apoptosis. Treatment with hsp27-specific antisense oligonucleotides prevented hsp27 protein expression and abolished the protective effects of heat shock and estrogen treatment on TNF alpha-induced apoptosis. Hsp27 is a determinant of osteoblast apoptosis, and estrogen treatment increases hsp27 levels in cultured osteoblastic cells. Hsp27 contributes to the control of osteoblast apoptosis and may be manipulated by estrogenic or alternative pathways for the improvement of bone mass.
Subject(s)
Apoptosis , Cell Death , Cell Line , Estradiol , Estrogens , Hot Temperature , HSP27 Heat-Shock Proteins , In Situ Nick-End Labeling , Indicators and Reagents , Oligonucleotides, Antisense , Osteoblasts , RNA, Messenger , Shock , Transferases , Tumor Necrosis Factor-alphaABSTRACT
Subject(s)
Humans , Congenital Abnormalities , Dentofacial Deformities , Esthetics , Lip , Nose , Orthognathic Surgery , Osteotomy , Prognosis , Retrospective StudiesABSTRACT
PURPOSE: We examined the relationships between the root curvatures of the mandibular third molars and their locations on the mandible, so that it might be contributed to elucidate the origin of the formation and development of the Korean mandibular third molar and help many clinicians to treat the mandibular third molars. MATERIALS AND EMTHODS: Panoramic radiographs of 237 patients who had been treated at Korea University Ansan Hospital from March, 2000 to April, 2001 and had bilateral mandibular third molars were examined and analyzed. RESULTS: There was no statistically significant difference between men and women (p < 0.05). But there were significantly fewer subjects with bilateral curved roots in bilateral third molars regardless of their status (p < 0.01) and were statistically significant differences between the groups with bilateral curved roots in the mandibular third molars with same status and with unilateral curved roots in the mandibular third molars with different status and between the groups with unilateral curved roots in the mandibular third molars with same status and with bilateral curved roots in the mandibular third molars with different status(p < 0.05). Subjects with bilateral curved roots in the mandibular third molars with bilateral partial impacted status and with bilateral curved roots in the mandibular third molars with bilateral complete eruption status were significantly fewer (p < 0.01) and subjects with the mandibular third molars with partial impacted and complete impacted status and with the mandibular third molars with complete erupted and partial impacted status had significantly fewer bilateral curved roots (p < 0.05). CONCLUSIONS: When we postulate that it is genetic and environmental factors that affect the root formation, it seems that environmental factors have more effects on the formation of the mandibular third molars than on that of the mandibular first and second molars.
Subject(s)
Female , Humans , Male , Korea , Mandible , Molar , Molar, ThirdABSTRACT
OR = 1mm). Serial lateral cephalometric radiographs were taken within 2 weeks preoperatively, within 1 week postoperatively and at a follow up period that ranged from 7 to 24 months postoperatively. All cephalometric radiographs were traced and digitized using the Quick ceph image Pro for analysis. And then, horizontal and vertical changes of reference points(B point, Pogonion, L1) were assessed by the linear measurements in millimeters on both axes. The results were as follows: 1. Mean horizontal relapses were 0.67+/-0.93mm (11%) at B point (P0.05). 3. The mean angular change of occlusal plane to SN was 0.24+/-1.19 (P>0.05) and that of mandibular plane to SN was 1.03+/-1.85 (P<0.05). 4. There was correlation between the extent of preoperative overbite and the postsurgical relapse at B point and Pogonion. 5. There was no correlation between the amount of surgically produced horizontal movement and the postoperative horizontal relapse at B point.